〔Abstract〕 Objective To investigate the effect of resveratrol(Rev) on proliferation and migration of ovarian cancer SKOV-3 cells by regulating IL-6/JAK2/STAT3 signaling pathway. Methods According to drug treatment, the cells were divided into SKOV-3 blank group(CON), Rev low concentration group(Rev-L), Rev medium concentration group(Rev-M) and Rev high concentration group(Rev-H). CCK-8 assay was used to detect the proliferation of SKOV-3 cells. Flow cytometry was used to detect the apoptosis of SKOV-3 cells. Healing assay was used to detect the migration of SKOV-3 cells. Transwell test was used to detect the invasion of SKOV-3 cells. Western blot assay was used to detect the expression levels of metastasis-related proteins, apoptosis-related proteins and IL-6/JAK2/STAT3 signaling pathway proteins in the each group. Results Compared with CON group, the apoptosis of SKOV-3 cells increased after Rev treatment, the proliferation, migration and invasion ability were inhibited; B lymphoma-2(Bcl-2) expression level was decreased; Bcl-2 family apoptotic proteins(Bax) and activated Caspase-3(cleaved Caspase-3) expression levels were increased; transfer related protein Ca2+dependent cell adhesion moleculue family-E(E-cadherin) expression level was increased, Ca2+dependent cell adhesion moleculue family-N(N-cadherin) and Vimentin expression levels were increased; and the activity of IL-6/JAK2/STAT3 signaling pathway decreased. Conclusion Rev can down-regulate the activity of IL-6/JAK2/STAT3 signaling pathway, promote the apoptosis of SKOV-3 cells, and inhibit the proliferation, migration and invasion of SKOV-3 cells.