〔Abstract〕 Objective To investigate the effects of Hashimoto′s thyroiditis(HT) on the concentration of glutamate(Glu) in the frontal lobe of pregnant rats and the changes of glutamate transporters. Methods After adaptively feeding for a week, 50 NOD female micewere randomly divided into normal control group(CON group, 20 mice) and Hashimoto thyroiditis group(HT group, 30 mice). HT mouse model was prepared by subcutaneous injection of porcine thyroglobulin in the tail root of HT group. After successful modeling, female mice in each group were caged with healthy NOD males to get pregnant. Serum thyroid stimulating hormone(TSH) levels were determined by ELISA. The levels of serum thyroid peroxidase antibody(TPOAb), thyroglobulin antibody(TgAb) and serum thyroid hormone triiodothyronine(T3) and thyroxine(T4) were determined by electrochemiluminescence immunoassay. The concentration of Glu in the frontal lobe of pregnant rats was detected by ELISA. Western blot assay was used to detect protein expression levels of vesicular glutamate transporter VGLUT 1 and excitatory glutamate transporter EAAT 2 in frontal lobe. RT-qPCR was used to observe the expression of VGLUT1 and EAAT2 mRNA in the frontal lobe of pregnant rats. Results ① Serum thyroid antibody levels(TPOAb, TgAb) in HT group were higher than those in CON group(P<0.05). There was no significant difference in serum T3, T4 and TSH between the two groups; ② The ELISA showed that the glutamate content in the frontal lobe of the HT group was higher than that in the CON group(P<0.05); ③ Western blot method showed that the expression of VGLUT 1 protein in HT group was lower than that in CON group(P<0.05), and the protein expression level of EAAT 2 in the two groups was not statistically significant;④ RT-qPCR showed that the mRNA expression of VGLUT 1 in the frontal lobe of HT group was lower than that in CON group(P<0.05). There was no significant difference in the expression level of EAAT 2 mRNA between the two groups. Conclusion HT can increase the content of Glu in the frontal lobe of pregnant mice, and decrease the expression of the glutamate transporter VGLUT 1 protein, suggesting that HT can cause glutamate metabolism disorders.