〔Abstract〕 Objective To investigate the effect of miR-223-3 p on cisplatin sensitivity of lung squamous cell carcinoma. Methods After increasing or decreasing(miR-223-3 p mimics, NC-mimics, miR-223-3 p inhibitor, NC-inhibitor) the content of miR-223-3 p in lung squamous carcinoma NCI-H520 and SK-MES-1 cells, the changes of miR-223-3 p in cells were detected by qRT-PCR, half inhibitory concentration(IC50) was detected by CCK-8 assay, apoptosis was detected by flow cytometry(four different treatments were designed:miR-223-3 p mimics+CDDP, NC-mimics, miR-223-3 p inhibitor+CDDP, NC-inhibitor), the migration ability of SK-MES-1 cells was detected by Transwell assay, the proteins of Bax, ZEB1 and E-cadherin in SK-MES-1 cells were detected by Western blot. Results The results of CCK-8 experiment showed that the IC50 of the miR-223-3 p mimics group was lower than that of the control group, and the IC50 of the miR-223-3 p group was higher than that of the control group. Flow cytometry apoptosis detection results showed that the apoptosis rate of miR-223-3 p mimics+ cisplatin(CDDP) group increased, while the apoptosis rate of miR-223-3 p inhibitor+ cisplatin(CDDP) group decreased. Western blot confirmed that ZEB1 protein, Bax and E-cadherin protein increased and decreased respectively after the content of miR-223-3 p in lung squamous cell carcinoma was increased or decreased. Conclusion miR-223-3 p affects the sensitivity of lung squamous cell carcinoma cells to cisplatin as an anticancer gene, and it can be an important molecular target for the treatment of cisplatin resistance in lung squamous cell carcinoma.