〔Abstract〕 Objective To detect quantitative results of hepatitis B surface antigen(HBsAg) in HBsAg weakly positive or both HBsAg and hepatitis B surface antibody(HBsAb) positive or anti-HBe antibody(HBeAb) and(or) anti-hepatitis B core antibody(HBcAb) positive in 5 items of hepatitis B detected by enzyme-linked immunosorbent assay(ELISA) and analyze their positive rates, thereby providing gist of interpreting commenting. Methods 56 cases of HBsAg weakly positive, 58 cases of both HBsAg and HBsAb positive and 61 cases of HBeAb and(or) HBcAb positive were screened out, and HBsAg quantitative value was re-detected by chemiluminescence immunoassay(CLIA). Results The positive rates of HBsAg in group 1±and 1±4+5+ re-detected by CLIA were 15.4% and 100%, respectively; the positive rates of HBsAg in group 1+2+ and 1+2+4+5+ re-detected by CLIA were 5% and 93.8%, respectively; the positive rates of HBsAg in group 5+ and 4+5+ re-detected by CLIA were 10.8% and 52.6%, respectively. The difference between the above two groups was statistically significant(P<0.01). Conclusion The results of HBsAb, HBeAb and HBcAb have important guiding significance for judging HBsAg results. HBeAb and HBcAb both being negative suggests the possibility of false positive of HBsAg in HBsAg weakly positive, both HBsAg and HBsAb positive ELISA patterns. Only HBeAb and HBcAb positive ELISA pattern suggests the possibility of false negative of HBsAg. Re-detection advice of HBsAg should be given according to the levels of HBeAb and HBcAb.