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Objective To investigate the killing effect of Zinc oxide nanoparticles(ZnO NPs) on kidney cancer Caki-1 cells and its correlation with autophagy. Methods Monolayer Caki-1 cells(2×103/well) were prepared in 96-well plates, treated with different concentrations(1、5、10、15、20、25 μg/ml) of ZnO NPs or treated with 15 μg/ml ZnO NPs for different time(6、12、24 h). Thiazole Blue(MTT) assay detected the inhibitory effect of ZnO NPs on Caki-1 cells. Subsequently, Western blot was used to detect the changes of autophagy effect of cells in different treatment groups. Finally, MTT method and Hoechst33342/PI staining method were used to determine the changes of cell viability after ZnO NPs were co-treated with autophagy inhibitors or enhancer. Results ZnO NPs had a dose and time dependent killing effect on Caki-1 cells. ZnO NPs also could elicit dose and time dependent autophagy effect. After the co-treatment of ZnO NPs and Wortmannin, the accumulation of LC3-Ⅱ decreased. The accumulation of LC3-Ⅱ increased after the co-treatment of ZnO NPs with Trehalose, an autophagy inducer. Meanwhile, after the co-treatment of ZnO NPs with the autophagy inhibitor wortmannin or chloroquine, cell viability was higher than that of ZnO NPs treated alone.However, when the cells were treated with ZnO NPs and Trehalose, the cell viability was further reduced. Conclusion ZnO NPs has a dose and time dependent cytotoxic effect on Caki-1 cells, and ZnO NPs can elicit pro-death autophagy effects in Caki-1 cells. Inhibition of autophagy can reduce cell death, and enhancement of autophagy can increase cell death. ZnO NPs can promote the formation of autophagosomes instead of inhibiting the degradation of autophagic substrates.

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Objective To investigate the potential mechanism of lipopolysaccharide(LPS)′s effect on inflammatory response of mouse glomerular endothelial cells(mGECs). Methods Isolation and culture of mGECs, CCK-8 assay and Western blot were used to determine the optimum concentration of LPS. mGECs were stimulated with LPS and incubated with JAK2 siRNA, STAT3 siRNAin vitro. The mRNA expression and secretion levels of tumor necrosis factor(TNF-α) and interleukin-6(IL-6) were detected by ELISA and qRT-PCR. JAK2 and STAT3 protein expressions and the phosphorylation level were detected respectively by Western blot. Results Compared with the control group, the expressions of JAK2 and STAT3 phosphorylation in mGECs increased in a concentration-dependent manner after LPS stimulation(P<0.05). The secretion of TNF-α, IL-6 in cell culture medium markedly increased(P<0.05). JAK2 siRNA and STAT3 siRNA could significantly inhibit the overexpression of TNF-α and IL-6 induced by LPS(P<0.05). Conclusion The JAK2/STAT3 signaling pathway is involved in the development of LPS-induced mGECs inflammation.

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Objective To explore the role of miR-146 a-5 p in the drug resistance mechanism of small cell lung cancer(SCLC). Methods The expression levels of miR-146 a-5 p in drug-resistant SCLC cell lines(H69-AR) and sensitive cell lines(H69) were tested by qRT-PCR to explore the correlation between miR-146 a-5 p and SCLC drug resistance. MiR-146 a-5 p was upregulated or inhibited by lentivirus vector stably transfected with miR-146 a-5 p mimic or miR-146 a-5 p inhibitor, respectively. The biological effects of miR-146 a-5 p on SCLC cell lines were verified by CCK-8 method and Nick experiment. The target gene of mir-146 a-5 p was predicted by searching related literatures and using the most authoritative software in biology. The correlation between miR-146 a-5 p and the predicted target genes was detected by western-blot, real-time quantitative qPCR and other methods. Results The expression level of miR-146 a-5 p in H69-AR compared with H69 cell line was(1 ∶22.70)(P<0.05). The potential target genes of miR-146 a-5 p were predicted by biological software to be interleukin-1 receptor-related kinase 1(IRAK1) and tumor necrosis factor receptor-related factor 6(TRAF6). The expression levels of mIRAK1 and mTRAF6 in H69-AR relative to H69 cells were significantly different(4.92 ∶1.00 and 2.76 ∶1.00)(P<0.05). After transfection of miR-146 a-5 p mimic in H69 AR cell line, compared with the control group, the cell inhibition rate increased, the migration rate slowed down, and the expression levels of mIRAK1 and mTRAF6 decreased to(1.00 ∶14.55) and(1 ∶3.20), respectively.The expressions of IRAK1 and TRAF6 decreased to(0.24 ∶1.00) and(0.31 ∶1.00)(P<0.05). After transfection of miR-146 a-5 p inhibitor in H69 cell line, compared with the control group, the cell inhibition rate decreased and the migration rate increased. At the same time, the expression levels of mIRAK1 and mTRAF6 increased to(10.86 ∶1.00) and(4.64 ∶1.00), respectivelywhile the expressions of IRAK1 and TRAF6 increased to(4.76 ∶1.00) and(3.96 ∶1.00), respectively(P<0.05). Conclusion MiR-146 a-5 p inhibits SCLC resistance by IRAK1 and TRAF6 genes.

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Objective To investigate the effects of electro-acupuncture on learning and memory and Bcl-2/Bax expression in hippocampal CA1 region of post-traumatic stress disorder(PTSD)-like rats. Methods Forty-eight healthy and clean male SD rats were randomly divided into four groups: control group, model group, electro-acupuncture group and electro-acupuncture control group, 12 rats in each group. The PTSD model of rats was established by the internationally recognized single prolonged stress( SPS) method. After 6 h of modeling,the rats in the electro-acupuncture group and electro-acupuncture control group were given acupuncture treatment. The open field test and Morris water maze were used to test the emotional arousal level and learning and memory ability of rats. The morphological changes of hippocampal CA1 region were observed by HE staining. Then the expression of Bcl-2/Bax protein in hippocampal CA1 region was detected by Western blot. Results Compared with the model group,the standing and crossing times of the control group and the electro-acupuncture group increased,the average incubation period and crossing latency shortened,the number of crossing times increased,and the difference was statistically significant( P<0. 05). The results of HE staining showed that compared with the control group,the hippocampal neurons in CA1 area of the model group were disorderly arranged and had an unusual structure,and the hippocampal neurons in CA1 area of the electro-acupuncture group were arranged in order and the structure tended to be normal. Compared with the control group,the expression of Bcl-2/Bax protein in CA1 region of hippocampus in the model group increased,and the expression of Bcl-2/Bax protein in CA1 region of hippocampus in the electro-acupuncture group decreased compared with the model group,and the difference was statistically significant(P<0. 05). Conclusion Electro-acupuncture can improve the learning and memory ability of PTSD-like rats,which may be related to the down-regulation of Bcl-2/Bax protein expression in CA1 region of hippocampus.

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Objective To study the expression of galactose agglutinin-1(galectin-1) in endometrium of Hashimoto's thyroiditis rats during estrous cycle. Methods A total of 40 female mice aged 8～9 weeks were selected and divided into observation group and control group by random number table method, with 20 mice in each group. In the observation group, pig thyroglobulin was injected subcutaneously at the tail root to establish Hashimoto's thyroiditis model. Mice serum thyroglobulin antibodies(TgAb), thyroid peroxidase antibody(TPOAb), triiodothyronine(T3),tetraiodothyronine(T4), thyroid stimulating hormone(TSH) level of two groups were compared respectively. The endometrial thickness,galectin-1 mRNA expression, and the average optical density(AOD) of galectin-1 protein in each period of estrus cycle were also compared. The levels of tumor necrosis factor-α(TNF-α) and interleukin-17 A(IL-17 A) were compared between the two groups. Results Compared with the control group, serum TgAb and TPOAb levels in the observation group increased(P<0.05), and there was no statistically significant difference in thyroid hormone levels(P>0.05). In the estrous phase, the endometrial thickness increased(P<0.05) while the expression of galectin-1 protein decreased(P<0.05). The mRNA level of galectin-1 decreased(P<0.05) during the estrous cycle, and the expressions of TNF-α and IL-17 a in the uterine tissue homogenate decreased(P<0.05). Conclusion The endometrium of Hashimoto's thyroiditis rats becomes thicker and the content of galectin-1 decreases during estrus. Uterine tissue homogenate TNF-α and IL-17 a decrease.

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Objective To explore the effects of pituitary adenylate cyclase activating polypeptide(PACAP) and recombinant PACAP(PACAP-PTD) containing protein transduction domain on thymus function in D-galactose(D-Gal)-induced immunosenescence mice, and to clarify its role in delaying immune aging. Methods Thirty male BALB/c mice were randomly divided into control group and model group, and 5 rats in the control group were injected subcutaneously with 500 mg/kg normal saline per day. The model group was injected with the same amount of D-Gal solution. After continuous injection for 42 days, 18 rat in the model group were randomly selected as PACAP-PTD group, PACAP group and D-Gal group, 6 for each group. The PACAP group and the PACAP-PTD group were intraperitoneally injected with 12.5 ml/kg PACAP and PACAP-PTD solution, and the D-Gal group was injected with the same amount of normal saline. Real-time PCR was used to detect initial T cell markers(sjTREC) in the thymus and spleen, the expression of the thymus-associated genes LIM domain only 2(LMO2), interleukin-7(IL-7) and forkhead box protein N1(Foxn1). Flow cytometry was used to detect the phenotypic changes of T lymphocytes CD3,CD4 and CD8 in whole blood of mice. Results The content of sjTRECs in T lymphocytes of mouse thymus and spleen: D-Gal groupP<0.01). Compared with the D-Gal group, PACAP and PACAP-PTD could up-regulate the expression of IL-7 and Foxn1(P<0.01), and down-regulate the expression of LMO2(P<0.01);moreover,PACAP-PTD up-regulated the expression of Foxn1 and IL-7 more significantly than PACAP(P<0.01). Ratio of CD3+CD4+/CD3+CD8+in mouse whole blood T lymphocyte subsets: D-Gal groupP<0.01). Conclusion PACAP and PACAP-PTD significantly enhance the initial T cell production and export function of mouse thymus, keep the diversity of peripheral T lymphocyte bank, up-regulate the expression of thymus function and statE-related genes, expand the thymus microenvironment, enhance cellular immune function and restore degraded thymic function, which have the effect of delaying aging and immune protection.

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Objective To investigate the effects of calcitonin gene-related peptide(CGRP) on osteogenic differentiation and angiogenesis of human periodontal ligament stem cells(hPDLSCs) in vitro. Methods hPDLSCs were isolated and cultured in vitro and identified by flow cytometry. Different concentrations of CGRP were administered,and CCK-8 method was used to detect the effect of CGRP on the proliferation of h PDLSCs. mRNA and protein expression of alkaline phosphatase( ALP),core binding factor 2( RUNX2),osteocalcin( OCN) and vascular endothelial growth factor( VEGF) were detected by RT-PCR and Western blot. ALP staining and alizarin red staining and semi-quantitative analysis were performed on the 7 th and 21 st day of osteogenic induction. Results The cells isolated from human periodontal ligament tissue were identified to be consistent with the characteristics of mesenchymal stem cells. Compared with the 0 mol/L group,CGRP in the 10-8 mol/L group and 10-9 mol/L group promoted the proliferation of h PDLSCs( P<0. 05),and simultaneously increased the expression of ALP,RUNX2,OCN,VEGF gene and protein( P<0. 05). ALP and alizarin red staining and semi-quantitative analysis showed that CGRP in the 10-8 mol/L group and 10-9 mol/L group promoted the expression of ALP and formation of calcium nodules in osteogenic differentiationof h PDLSCs compared with the 0 mol/L group. Conclusion Specific concentrations of CGRP promote osteogenic differentiation and angiogenic ability of h PDLSCs in vitro.

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Objective To explore the effects of neurotropin(NTP) on Notch signaling pathway and hippocampus neurons autophagy in Alzheimer′s disease(AD) model rats. Methods 45 SD rats were randomly divided into control group, model group and NTP group equally. Model group and NTP group were intraperitoneally injected with D-galactose(100 mg/kg) and hippocampus injected with Aβ1-42(5 μl) to establish AD rat models. NTP group was intraperitoneally injected with NTP(1.2 Nu/kg) for 2 weeks intervention. The learning and memory ability of rats in each group was detected by Morris water maze. The pathological changes of hippocampus tissues were observed by HE staining. The apoptosis of hippocampus neurons was detected by TUNEL method. The autophagy of hippocampus neurons was observed by electron microscope. Western blot was performed to detect Notch signaling pathway and the expressions of Caspase-3, Bcl-2, Bax, Beclin1 and LC3-Ⅱ/LC3-Ⅰ. Results Compared with the control group, the learning and memory ability of the model group and NTP group significantly decreased(P<0.05), while apoptosis rate of hippocampus tissue cells significantly increased(P<0.05). The expressions of Bcl-2, Beclin-1, Notch1, NICD and Hes5 proteins were significantly down-regulated, while the expressions of Caspase-3 and Bax were significantly up-regulated(P<0.05). Compared with the model group, learning and memory ability of the NTP group significantly increased(P<0.05), the expressions of Bcl-2, Beclin-1, LC3-Ⅱ/LC3-Ⅰ, Notch1, NICD and Hes5 in hippocampus tissues were significantly up-regulated(P<0.05), while the expressions of Caspase-3 and Bax protein were significantly down-regulated(P<0.05). HE staining showed that pathological changes in hippocampus tissues of rats in the NTP group were significantly reduced. Conclusion NTP can induce hippocampus neurons autophagy, improve learning and memory ability of AD rats and protect brain tissue damage by regulating Notch signaling pathway.

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Objective To observe the protective effect of pretreatment with rosiglitazone(RSG) on lipopolysaccharide(LPS)-induced acute kidney injury(AKI) and explore the anti-inflammatory mechanism. Methods Thirty-two male ICR mice were randomly divided into four groups: Control group, RSG group, LPS group,RSG+LPSgroup. In LPS group, mice were intraperitoneally injected with single dose of LPS(2 mg/kg). In RSG+LPS group, mice were pretreated with RSG(10 mg/kg) at 72, 48, 24 and 1 h before LPS injection. All mice were sacrificed 6 h after LPS injection. Kidneys and sera were collected. Some kidneys were used for HE staining. Serum inflammatory cytokines were detected by ELISA. The expression of renal inflammatory cytokines was analyzed using real-time RT-PCR. Renal NF-κB p65 phosphorylation was measured through Western blot. Results RSG pretreatment ameliorated LPS-induced acute kidney injury and inflammatory cell infiltration. Moreover,RSG pretreatment inhibited LPS-induced elevation of serum KC and TNF-α． In addition,RSG pretreatment alleviated LPS-induced upregulation of proinflammatory cytokines( TNF-α and IL-1β) and chemokines( KC and Mcp-1). Finally,RSG pretreatment suppressed LPS-induced renal NF-κB p65 phosphorylation. Conclusion RSG protects against LPSinduced acute kidney injury through inhibiting renal inflammation.

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Objective To observe cytology differences of liver stem cells(LSCs) in non-inflammatory proliferation environment and inflammatory proliferation environment by establishing non-inflammatory LSCs proliferation model and inflammatory LSCs proliferation model. Methods 2-acetaminofluorenced combined two thirds partial hepatectomy protocol was established as non-inflammatory LSCs proliferation model. 2-acetaminofluorence combined carbon tetrachloride protocol was established as inflammatory LSCs proliferation model. Liver histological changes were observed by hematoxylin and eosin staining. The expressions of EpCAM, CD133 and OV6 of LSCs were detected by immunohistochemistry, and average optical density values were measured by Image-Pro Plus 6.0. The ultrastructure changes of LSCs were observed by transmission electron microscopy. Results Non-inflammatory LSCs proliferation model and inflammatory LSCs proliferation model were established successfully. HE staining:non-inflammatory LSCs proliferation model rats' liver were absent with inflammatory cells infiltration; inflammatory LSCs proliferation model rats' liver were fibrosis formation period with large number of inflammatory cells infiltration. Immunohistochemical: EpCAM-positive, CD133-positive, and OV6-positive LSCs were found both in non-inflammatory and inflammatory LSCs proliferation model, the expression levels of EpCAM, CD133, OV6 of inflammatory LSCs proliferation model were significantly higher than those of non-inflammatory LSCs proliferation model(P<0.05). Transmission electron microscopytype: Ⅰ,Ⅱ, and Ⅲ of LSCs were found both in non-inflammatory and inflammatory LSCs proliferation model, however, there were more heteromorphic LSCs in inflammatory LSCs proliferation model than those in non-inflammatory LSCs proliferation model(P<0.05). Conclusion There are more EpCAM+, CD133+, OV6+LSCs in inflammatory LSCs proliferation model compared with non-inflammatory LSCs proliferation model, and there are LSCs with abnormal subcellular structure in the early stage of hepatitis.

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Objective To investigate the expression of Rac1 and Cdc42 in rhabdomyosarcoma(RMS) cells and their effect on proliferation, invasion, migration and apoptosis of RMS cells. Methods The expressions of Rac1 and Cdc42 were detected by qRT-PCR and Western blot in RMS cells and human skeletal muscle cells. The Rac1 and Cdc42 overexpression plasmids were constructed, and transfection efficiency was detected by qRT-PCR and Western blot. The effects of Rac1 and Cdc42 on the proliferation, invasion, migration and apoptosis of RMS cells were detected by CCK-8 assay, transwell system, wound-healing assay and flow cytometry assay. Results The expression levels of Rac1 and Cdc42 in RMS cells were higher than those in normal skeletal muscles cells. RMS cells transfected with Rac1 or Cdc42 overexpression plasmid had higher intracellular Rac1 and Cdc42 levels than those transfected with the empty vector(EV). Compared with the control group, overexpressing Rac1 and Cdc42 in RMS cells remarkably promoted proliferation, invasion, migration and anti-apoptosis. Conclusion Rac1 and Cdc42 are highly expressed in RMS cells and promote the malignant biological behavior of RMS cells by affecting the proliferation, invasion, migration and anti-apoptotic ability of RMS cells.

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Objective To investigate the therapeutic effects of human placental mesenchymal stem cells(hPMSCs) on asthmatic rats and the effects of Notch signaling pathway related factors. Methods Thirty SD rats were randomly divided into three groups: control group, asthma model group(OVA group) and hPMSCs transplantation group(hPMSCs group), 10 rats/group. The morphological changes of lung tissue were observed by HE staining. The inflammatory factors in rat serum were detected by ELISA. The Notch1, Notch2, Notch3 and Jagged1 mRNA and protein in rat lung tissue were detected by RT-qPCR and Western blot. Results HE staining results showed that compared with OVA group, the lumen of hPMSCs group was slightly constricted and inflammatory cell infiltration was alleviated. ELISA results showed that serum immunoglobulin E(IgE) and interleukin 4(IL-4) levels in OVA group were higher than those in control group(P<0.05), while interferon-γ(IFN-γ) level was lower(P<0.05).Compared with OVA group, the levels of IgE and IL-4 in serum of hPMSCs group decreased(P<0.05), while the levels of IFN-γ in serum increased(P<0.05). The results of RT-qPCR and Western blot showed that the expressions of Notch1, Notch2 and Jagged1 mRNA and protein in lung tissue of OVA group were higher than those of control group, while the expression of Notch3 mRNA and protein was lower. Compared with OVA group, the expression of Notch1, Notch2, Jagged1 and Notch3 mRNA and protein in hPMSCs group decreased, while the expression of Notch3 mRNA and protein increased. Conclusion hPMSCs can inhibit the inflammatory response of asthmatic rats by regulating Notch signaling pathway and play a role in the treatment of asthma.

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Objective To explore effects of serum containing Bailing capsules and reduced glutathione(GSH) on proliferation of rat interstitial fibroblasts NRK-49 F cells and expression level of transforming growth factor-beta1(TGF-β 1) and sma-and mad-related protein(Smad). Methods Rats in unilateral ureteral obstruction group were applied to make drug-containing serum(1.5 g/kg Bailing capsules combined with 300 mg/kg GSH) and drug-free serum. The cells in logarithmic growth phase were divided into normal control group, model group(10 ng/ml TGF-β1), negative control group(10 ng/ml TGF-β1+10% drug-free serum) and drug intervention group(10 ng/ml TGF-β1+10% drug-containing serum). The cell morphology of each group was observed under microscope. The cell proliferation of each group was detected by CCK8. The cell cycle distribution of each group was detected by flow cytometry. The expression of TGF-β1/Smad signaling pathway transcription level of each group was detected by RT-PCR. Western blot was performed to detect expression of TGF-β1/Smad signaling pathway protein level in each group. Results Compared with blank control group, proliferation activity of NRK-49 F cells in model group and negative control group significantly increased(P<0.05), cells in G0/G1 phase significantly decreased(P<0.05) and cells in S phase significantly increased(P<0.05). Expressions of proliferating cell nuclear antigen(PCNA), Cyclin D1,Collagen Ⅰ, α-smooth muscle actin(α-SMA), phosphorylation of Smad2/Smad2(p-Smad2/Smad2) and p-Smad3/Smad3 were significantly up-regulated(P<0.05). Compared with negative control group, proliferation activity of NRK-49 F cells in drug intervention group significantly decreased(P<0.05),cells in G0/G1 phase significantly increased(P<0.05) and cells in S phase significantly decreased(P<0.05). Expressions of PCNA, Cyclin D1, Collagen I, α-SMA, p-Smad2/Smad2 and p-Smad3/Smad3 were significantly down-regulated(P<0.05). Conclusion Serum containing Bailing capsules and reduced glutathione may inhibit renal interstitial fibrosis by inhibiting expression of TGF-β1/Smad.

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Objective To investigate the effect of acid-sensitive ion channel-1 a on the expression of endoplasmic reticulum stress-related protein in rats with acute lung injury induced by lipopolysaccharide(LPS).Methods 40 SPF male Sprague Dawley rats weighing 180～220 g were randomly divided into 4 groups(10 in each group): Control group, Model group, Amiloride group and Dexamethasone(Dex) group. The pathological changes of lung tissue were observed in rats, and the expression of interleukin-6(IL-6) in serum was detected by ELISA. The expres-sion levels of glucose-regulated protein 78(GRP78), C/EBP-homologous protein(CHOP), Inositol-requiring kinase 1( IRE-1) and X box binding protein 1( XBP-1) were detected by Real-time quantitative PCR. GRP78,ccaspase-12,IRE-1α and XBP-1 in lung tissue were detected by Western blot. Results Compared with the Control group,lung tissues in the Model group showed alveolar collapse and interstitial edema; the expression of IL-6 was up-regulated( P<0. 01). The expression of indicators related to lung tissue decreased in the amiloride group and the Dex group. Western blot results showed that the protein expression of endoplasmic reticulum stress-related genes in the Model group was increased compared with the Control group,and the protein expression of endoplasmic reticulum stress-related genes in the Amiloride group was lower than that in the Model group( P<0. 01). Real-time quantitative PCR results showed that the mRNA expression of endoplasmic reticulum stress-related genes in the Model group was increased compared with the Control group,and the mRNA expression of endoplasmic reticulum stress-related genes in the Amiloride group was lower than that in the Model group( P<0. 01). Conclusion Upregulation of ASIC1 a in LPS-induced acute lung injury rats may mediate endoplasmic reticulum stress response.

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Objective To study the optimal energy density of Q-switched Cr, Er:YSGG laser,which wavelength is 2.79 μm, on enamel at nanosecond time scale by exploring the laser ablation threshold and the optimal energy density of laser treatment of enamel and recording the change of enamel temperature during the process. Methods Q-switched nanosecond pulsed Cr, Er:YSGG laser was used to treat the surface of 5 groups′enamel samples with different energy density. The surface morphology was observed by Field emission scanning electron microscopy. Subsequently Ablation threshold and energy density of each group were calculated. Energy dispersive X-ray spectroscopy was employed to detect the chemistry ingredients. Real-time monitoring and recording of temperature changes during the action of Cr, Er:YSGG laser were also recorded by a high-performance infrared camera. Results The laser ablation threshold and the optimal energy density interval of enamel with laser treated were founded and the change of enamel surface temperature corresponding to different laser energy density at low energy was collected. Conclusion The treatment of enamel with Q-switched nanosecond pulse Cr,Er:YSGG laser is fast and effective, clean and pollution-free,which avoids thermal damage to enamel and surrounding tissues including dental pulp,improves the treatment safety and provides research basis for the clinical application of nanosecond laser in oral cavity.

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Objective To investigate the theoretical basis and new treatment methods of specific anti-Porphyromonas gingivalis yolk antibody liposome(anti-Pg-IgY liposome) in clinical adjuvant treatment of chronic periodontitis and improve its efficacy by observing its entrapment efficiency and the effect of in vitro sustained release. Methods Anti-Pg-IgY liposomes were prepared by thin film dispersion method. Zeta potential and particle size of anti-Pg-IgY liposomes were determined by dynamic light scattering particle size distribution instrument. Entrapment efficiency was determined by dialysis method combined with Elisa.In vitrorelease rate of anti-Pg-IgY liposomes in 0.9% physiological saline was investigated, and its quality was evaluated by transmission electron microscope. Results The Zeta potential of liposome was(-38.55±0.16)mV, the average particle size was(82.48±2.3) nm, the polydispersity index was(0.313±0.01), the entrapment efficiency of liposome was 40%, the cumulative release rate in 0.9% physiological saline was less than 20% within 4 h, about 50% within 24 h, and the release rate in 108 hours was 79.9%. Conclusion Anti-Pg-IgY liposome has high entrapment efficiency,nanometer size and good stability. It is expected to realize long-cycle sustained release in periodontal pocket.

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Objective To explore the mechanism of chronic prenatal stress inducing depression in offspring male rats.Methods Thirty pregnant rats were randomly divided into the control group and the chronic unpredictable mild stress group(CUMS). After the rats were born, male offspring rats were randomly divided into the control + Solvent group(CON group), the control + CRHR1 antagonist group(CON+ANT group), the CUMS + CRHR1 antagonist group(CUMS+ANT group) and the CUMS+Solvent group(CUMS group). Solvent or CRHR1 antagonist were administered by lateral ventricle injection to 10～30-day-old rats. Then we observed depressive-like behaviors, number and morphology of hippocampal CA3 neurons, the level of corticotrophin-releasing hormone(CRH) in the hippocampus, mammalian target of rapamycin(mTOR) and p-mTOR(Ser2448) protein expression. Hippocampal slices of 10-day-old rats were cultured in vitro, and slices were divided into the control group(Control group),1.0 μmol/L CRH+different dose of CRHR1 antagonist group and 1.0 μmol/L CRH+solvent control group(CRH group).Western blot was used to observe the expression of mTOR protein.Results Compared with the CON group,the offspring rats in CUMS group had more motion time in forced swimming test( P<0. 05) and less sugar preference rate in the sugar preference test( P<0. 05). The hippocampus CRH concentration increased( P<0. 05) and the neuropathological changes were characterized by decreased neuron number,loosed neurons and the soma condensation in CA3 field of hippocampus. Moreover,the expression of m TOR and p-m TOR both decreased in hippocampus( P<0. 05). Compared with the CUMS group,the offspring rats in CUMS + CRHR1 antagonist group had less motion time in forced swimming test( P<0. 05) and more sugar preference rate in the sugar preference test( P<0. 05). The level of CRH in hippocampus was down-regulated,the neuropathological damage in hippocampus CA3 region was improved and the expression of m TOR and p-m TOR both increased( P<0. 05). The results of hippocampal slicesculture showed that the expression of m TOR decreased more in CRH group than that in Control group( P<0. 05). Low,middle and high concentration of CRHR1 antagonist could upregulate the decreased level of m TOR induced by CRH( P<0. 05). Conclusion Depression of male offspring caused by chronic prenatal stress may be associated with the increased level of CRH in the hippocampus which inhibit the expression of m TOR protein and cause the neuropathological damage in the CA3 field of hippocampus.

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Objective To provide a new monitoring index for clinical diagnosis and prognosis evaluation of breast cancer, and to provide a guiding theoretical basis for further research and treatment program formulation of breast cancer by studying the expression of Cbl Proto-Oncogene C(CBLC) in breast cancer. Methods The gene expression spectrum matrix(FPKM) of 1104 breast cancer tissues and 113 adjacent tissues were downloaded from TCGA of USCS Xena(https://xenabrowser.net). The expression differences of CBLC gene in breast cancer molecular classification, histopathological type, TNM stages and tumor stages and the relationship of prognosis were analyzed. The expression of CBLC in 30 tumor tissues or para-carcinoma tissues were observed by immunohistochemical staining. MCF7 breast cancer cells were used for scratch test, Transwell test and CCK-8 test, and the effects of CBLC on proliferation and migration were observed. Results The expression level(P=2.31×10-20) and H-Score(P=0.014)of CBLC in the breast cancer group was higher than that in the para-carcinoma group. CBLC expression in Luminal A(P=1.68×10-4) and mucinous carcinoma(P=4.00×10-5) breast cancer was the highest among different breast cancer molecular types(P=1.68×10-4). The expression level of CBLC decreased from stage Ⅰ to stage Ⅱ in breast cancer(P=0.049 4). The survival rate of breast cancer patients in the high CBLC expression group was higher than that in the low CBLC expression group on a median basis(P=0.054). The high expression group of CBLC could inhibit the proliferation and migration of breast cancer cells. Conclusion The expression of CBLC in various breast cancer types and stages is significantly different, and it mainly expresses in Luminal A breast cancer which is low malignant molecular type, mucinous carcinoma which is high differentiated histopathological type and early breast cancer. It is positively correlated with survival and prognosis of breast cancer patients, and it can inhibit the migration and proliferation of breast cancer. CBLC may be a potential target for new molecular diagnosis or targeted treatment of breast cancer.

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Objective To explore effects of extracorporeal shock wave(ESW) combined with platelet rich plasma(PRP) on bone healing in rats with osteoporotic fracture.Methods Osteoporosis rat models were established by ovariosteresis(OVX). 50 female 3 months old SD rats were randomly divided into control group(Control), OVX group, OVX+ESW group, OVX+PRP group and OVX+ESW+PRP group, 10 cases in each group. In Control group, the same amount of ovarian surrounding tissue was excised. The right femur fracture was conducted on rats in the above groups, and the rats were given intramedullary fixation with Kirschner wire. The effects of ESW combined with PRP on bone healing in rats with osteoporotic fracture were observed. And the action mechanism was explored.Results After OVX, bone mineral density of rats was(0.22±0.07)g/cm2and significantly lower than that in Control group(P<0.05). Imaging showed that fracture line was clear in OVX+ESW+PRP group, there were large number of bone callus tissues formation, and fracture healing was better than that in OVX+ESW group and OVX+PRP group. HE staining showed that after treatment, bone trabecula was thicker and its number got more in OVX+ESW+PRP group. And arrangement was orderly, histopathological improvement was better than that in OVX+ESW group and the OVX+PRP group. After OVX rats were treated with ESW and PRP, in maximum load at fracture site,the expression of bone morphogenetic protein-2( BPM-2),runt-related transcription factor 2(RUNX2) and zinc finger transcription factor(OSX) in bone callus tissue increased,while the levels of serum bone alkaline phosphatase( BALP),bone gla protein( BGP) and procollagen type Ⅰ carboxy-terminal peptide(PICP) decreased(P<0. 05). And the effect was better than that in OVX + ESW group and OVX + PRP group.Conclusion ESW combined with PRP can promote bone healing in rats with osteoporotic fracture,which may be related to down-regulation of BALP,BGP and PICP levels,and up-regulation of BPM-2,RUNX2 and OSX expression.

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Objective To analyze the distribution characteristics of common capsular serotypes and virulence genes ofKlebsiella pneumoniaewith positive blood flow infection in the First Affiliated Hospital of Anhui Medical University, and to explore the correlation between virulence genes and CRISPR-CAS system. Methods 61 strains ofKlebsiella pneumoniaewere collected from blood culture bottles and identified asKlebsiella pneumoniae. The susceptibility ofKlebsiella pneumoniaeto commonly used antibiotics was detected by disk diffusion method and ViteK-compact 2 instrument.Klebsiella pneumoniaewith high mucus type were screened by wire drawing test. Six common capsular serotypes(K1, K2, K5, K20, K54, K57), seven virulence-related genes(rmpA, aerobactin, alls, Kfu, wcaG, magA, fimH) and three CRISPR-CAS system genes(CRISPR-1, CRISPR-2, CAS1) were detected by polymerase chain reaction(PCR). Results The serotypes of capsule were mainly K1 and K2, and the detection rates were 19.7% and 13.1% respectively. K5, K20 and K57 serotypes were detected in one case while K54 serotypes were not detected. The detection rates of virulence genes rmpA, aerobactin, alls, Kfu, wcaG, magA and fimH were 32.8%, 23.0%, 9.8%, 31.1%, 19.7%, 19.7% and 98.4%, respectively.14 strains were screened for high mucus phenotype. 11 strains were detected as positive by CRISPR-CAS system and the detection rate was 18%. Compared with the negative strains of CRISPR-CAS system, the virulence gene carrying rate of CRISPR-CAS system was higher, and the difference was statistically significant by χ2test(P<0.05). Conclusion K1 and K2 are the main serotypes ofKlebsiella pneumoniae. The virulence genes mainly exist in the strains with high mucus phenotype. The virulence gene carrying rate of CRISPR-CAS positive strains is high.

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Objective To investigate the expression of SENEX in diffuse large B-cell lymphoma(DLBCL) and its effect on the recurrence of DLBCL and its possible mechanism. Methods Western blot and qRT-PCR were used to detect SENEX gene and protein expression in 3 patients with newly diagnosed DLBCL(newly diagnosed group), 3 patients with relapsed DLBCL(recurrent group) and 3 patients with complete remission of DLBCL(complete remission group). SENEX in DLBCL cell OCI-LY8 were silenced with SiRNA transfection technology and the efficiency of transfection was verified by Western blot and qRT-PCR. The cell proliferation was measured by the CCK-8 analysis. The concentration of free transcription factor binding to the adenovirus E2 promoter(E2 F) in cell supernatant was measured by ELISA. Western blot was used to detect expression of p16 and retinoblastoma(Rb)/phosphorylation retinoblastoma(pRb). Results SENEX and protein levels increased significantly in patients with relapsed DLBCL; SENEX expression in silencing DLBCL cells inhibited the proliferation of DLBCL cells, E2 F activation and Rb activation. Conclusion The SENEX gene promotes proliferation and recurrence of DLBCL by activating the Rb/E2 F1 pathway.

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Objective To explore the relationship of two single nucleotide polymorphisms(SNPs), rs150846 and rs8065080, of TRPV1 gene with childhood asthma of Kazakh and Han in Xinjiang. Methods The participants enrolled were 99 inpatients and outpatients(case group) who received treatment of Pediatrics, including 52 Kazakhs and 47 Hans. 77 healthy controls(control group) underwent physical examination, including 39 Kazakhs and 38 Hans. All the children were taken 2 ml of peripheral venous blood, and DNA of the blood sample was extracted. The Massarray SNP typing technique was used to detect the polymorphisms of TRPV1 gene in peripheral venous blood cells. Results ① The patterns of genotype and allele frequencies of rs150846 at the TRPV1 locus had statistical difference between the groups(P<0. 05),but the patterns of genotype and allele frequencies of rs8065080 at the TRPV1 locus did not differ significantly between the groups(P>0.05). ② Among the Kazakh population, the patterns of genotype and allele frequencies of rs150846 at the TRPV1 locus had statistical difference between the groups(P<0.05). The allele A of rs150846 had a 1.941-fold(95%CI:1.037～3.636) increased risk of developing asthmatic diseases compared with those who had allele G. But the patterns of genotype and allele frequencies of rs8065080 at the TRPV1 locus did not differ significantly between the groups(P>0.05).③ Among the Han population, the patterns of genotype and allele frequencies of rs150846 and rs8065080 at the TRPV1 locus did not differ significantly between the groups(P>0.05). Conclusion The rs150846 gene polymorphism of TRPV1 gene could be associated with susceptibility to childhood asthma of Kazakh in Xinjiang.

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Objective To investigate the depressive state and the features of ecological executive function in children with epilepsy and explore the relationship between depression and executive function. Methods The research recruited 51 children with epilepsy as the experimental subjects and 48 typically developing children as control subjects. Both groups completed Children's Depression Inventory(CDI) and Behavior Rating Inventory of Executive Function(BRIEF). The children with epilepsy were divided into two groups according to the CDI scores: epilepsy with depression and epilepsy without depression. The BRIEF scores between the two groups were compared. Results ① Of all 51 children with epilepsy,16 had depression, resulting in a comorbidity rate of 31.37%, which is significantly higher than the control group(P<0.05). ② The scores of Global Executive Composite(GEC), Behavioral Regulation Index(BRI) and Metacognition Index(MI) of BRIEF [(54.10±9.64), 51(44,58),(55.00±8.84)] were significantly higher than those of the control group [(43.83±6.86), 41(40,44),(45.83±7.26)]respectively(P<0.05). ③ Multiple stepwise regression analysis showed that depression in children with epilepsy was negatively correlated with the effective control of epileptic seizure, and GEC was positively correlated with the number of drug types, and negatively correlated with the effective control of seizure(P<0.05). ④ Pearson's correlation test showed that the scores of BRIEF(including GEC, BRI, MI and all sub factors) had significantly positive correlations with the score of depression(r=0.385～0.597,P<0.05). Conclusion Children with epilepsy have depressive disorder and executive dysfunction compared with healthy controls. The patients with epilepsy and depression show more serious impairments of ecological executive function than those do not have depression. Depression and executive function in children with epilepsy have significant correlations.

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Objective To observe the expression of miR-122 and miR-155 in plasma of patients with viral hepatitis type B and analyze their correlation with inflammatory factors and cell cycle factors. Methods The expressions of miR-122 and miR-155 were determined by qRT-PCR. The expressions of interleukin(IL)-6, tumor necrosis factor(TNF)-α, P53, CyclinD1 were determined by ELISA. Results The results of qRT-PCR showed that expressions of miR-122 and miR-155 in the plasma of patients with viral hepatitis type B were high(P<0.05). During the course of continuous treatment, miR-122 in the patient's plasma increased first and then decreased, and miR-155 expression decreased. The expressions of miR-122 and miR-155 were positively correlated with alanine aminotransferase(ALT),aspartate aminotransferase(AST), IL-6, TNF-α and CyclinD1(P<0.01), and miR-155 had more obvious correlation. There was a negative correlation with p53(P<0.01). Conclusion The abnormal expression of miR-122 and miR-155 in plasma of patients with hepatitis B virus(HBV) is related to liver damage caused by HBV, which provides a new idea for the monitoring and treatment of liver damage caused by HBV.

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Objective To detect quantitative results of hepatitis B surface antigen(HBsAg) in HBsAg weakly positive or both HBsAg and hepatitis B surface antibody(HBsAb) positive or anti-HBe antibody(HBeAb) and(or) anti-hepatitis B core antibody(HBcAb) positive in 5 items of hepatitis B detected by enzyme-linked immunosorbent assay(ELISA) and analyze their positive rates, thereby providing gist of interpreting commenting. Methods 56 cases of HBsAg weakly positive, 58 cases of both HBsAg and HBsAb positive and 61 cases of HBeAb and(or) HBcAb positive were screened out, and HBsAg quantitative value was re-detected by chemiluminescence immunoassay(CLIA). Results The positive rates of HBsAg in group 1±and 1±4+5+ re-detected by CLIA were 15.4% and 100%, respectively; the positive rates of HBsAg in group 1+2+ and 1+2+4+5+ re-detected by CLIA were 5% and 93.8%, respectively; the positive rates of HBsAg in group 5+ and 4+5+ re-detected by CLIA were 10.8% and 52.6%, respectively. The difference between the above two groups was statistically significant(P<0.01). Conclusion The results of HBsAb, HBeAb and HBcAb have important guiding significance for judging HBsAg results. HBeAb and HBcAb both being negative suggests the possibility of false positive of HBsAg in HBsAg weakly positive, both HBsAg and HBsAb positive ELISA patterns. Only HBeAb and HBcAb positive ELISA pattern suggests the possibility of false negative of HBsAg. Re-detection advice of HBsAg should be given according to the levels of HBeAb and HBcAb.

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Objective To investigate the correlation between hyperuricemia and clinical features and Oxford pathology classification of IgA nephropathy on renal biopsy. Methods A retrospective analysis was performed on 289 patients diagnosed of primary IgA nephropathy by renal biopsy. According to the level of uric acid on renal biopsy, the patients were divided into two groups including 120 patients in the high uric acid group and 169 patients in the normal uric acid group. Clinical and histopathological data were collected to analyze the relationship between hyperuricemia and clinical features and Oxford classification on renal biopsy. Results The incidence of hyperuricemia in the 289 patients with IgA nephropathy was 41.5% on renal biopsy. Compared with the normal uric acid group, the high uric acid group had a higher incidence of hypertension. The levels of creatinine, urea nitrogen and 24-hour urinary protein were higher and the lipid metabolisms of triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein were more abnormal in the high uric acid group. Spearman correlation analysis showed that uric acid was closely related to mesangial proliferation(r=0.254,P<0.000 1), glomerular segmental sclerosis(r=0.145,P=0.013) and tubular atrophy or interstitial fibrosis(r=0.315,P<0.000 1). Multivariate logistic regression showed that hyperuricemia was an independent risk factor for mesangial proliferation and glomerular segmental sclerosis after adjustment of other clinical indicators. Three different models were established based on different clinical indicators. ROC curve combined diagnosis revealed that uric acid could improve the diagnostic ability of mesangial proliferation and glomerular segmental sclerosis. The more combined indicators, the greater the diagnostic efficiency. Conclusion Hyperuricemia is closely related to renal dysfunction and lipid metabolism disorder in IgA nephropathy on renal biopsy. At the same time, hyperuricemia is associated with renal pathological injury. As an independent risk factor for mesangial proliferation and glomerular sclerosis, hyperuricemia can improve the diagnostic value of mesangial proliferation and glomerular sclerosis in IgA nephropathy on renal biopsy.

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Objective To analyze the cause of drug resistance of hepatitis B virus by studying mutation sites the of HBV RT region under nucleoside(acid) analogues in Jiangxi Province. Methods Serum samples of 104 outpatients receiving NAs antiviral therapy for more than one year were collected, and fragments of possible drug-resistant mutation sites in HBV RT region were amplified. The levels of HBV DNA and ALT with poor response and salvage treatment for 1 month were collected to analyze the effect of salvage treatment. Nucleic acid and amino acid sequences were compared by BioEdit software package to analyze the main variant types in RT region of patients and their distribution with different genotypes. DataMonkey online software was used to analyze the selection pressure and positive selection sites of different genotypes. Results After salvage treatment, HBV DNA and ALT levels decreased, and HBV DNA levels decreased more significantly, but there was no difference among different genotypes. Of 104 patients, 87 were associated with drug resistancE-related mutations, 14(13.46%) were multi-site resistance mutations(3 or more sites); 7 of the remaining 17 patients did not detect the drug resistance mutation,10 were possible drug resistance mutation sites. M204 I + L180 M site mutation rate in genotype C was higher than that of genotype B,and the difference was statistically significant. Analysis of selection pressure showed that there was no difference in selection pressure between the two genotypes,and neutral selection was the main choice. Rt207 and rt229 were positive selection sites in genotype B,but not in genotype C. Conclusion Genotypes B and C are most common types in chronic hepatitis B patients in Jiangxi Province,and the M204 I locus variation is dominant.Positive selection sites are detected in Genotype B,so the widespread use of nucleoside analogues promote the emergence of new gene functions,and may also be the cause of multiple new drug resistance sites.

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Objective To investigate the occurrence, treatment and pregnancy outcome of multiple pregnancy afterin vitrofertilization and embryo transfer treatment(IVF-ET), and to provide reference for health care of iatrogenic multiple pregnancy and the prevention&treatment of artificial assisted multiple pregnancy complications. Methods Selecting 4 321 cases of patients with multiple pregnancy through treatment of in IVF-ET pregnancy, retrieving the patient information management system, counting the occurrence of iatrogenic multiplets and pregnancy outcomes, analyzing and comparing the indicators including the processing of multiple pregnancy and pregnancy outcomes according to whether the fetus was reducted or not. SPSS19.0 software was used for data analysis. The test methods mainly includettest, chi-square test, etc. Results ① The subjects were divided into two groups according to the chorionic nature of pregnancy: single chorionic multiple foetuses and independent chorionic multiple foetuses. There was no statistically significant difference in age, BMI, infertility type, transplantation cycle and embryo source between the two groups(P>0.05), and there were statistically significant differences in infertility years and causes between the two groups(P<0.05). ② The subjects were divided into three groups according to whether the fetus was reducted or not: natural fetus reduction, surgical fetus reduction, and non reduction. The rate of late abortion or induced labor in the Non Reduction group was higher than that in the Reduction group(χ2=12.426,P=0.002); the rate of premature birth in the Non Reduction group was higher than that in the Reduction group(χ2=196.038,P<0.001); the rate of cesarean section birth in the Non Reduction group was higher than that in the Reduction group(χ2=45.181,P<0.001); and the neonatal weight in the Non Reduction group was lower than that in the Reduction group(F=3.251,P<0.001). ③ The subjects were divided into two groups according to the number of continuous pregnancies: single and twin. There were statistically significant differences in pregnancy complications, preterm delivery rate, cesarean section rate and newborn birth weight between the two groups(χ2=156.648,P<0.001;χ2=94.572,P<0.001;χ2=32.042,P<0.001;t=14.928,P<0.001). Conclusion Iatrogenic multiple pregnancy has a high rate of pregnancy loss and a large number of maternal and infant complications, so iatrogenic multiple pregnancy should be avoided as far as possible. For patients with multiple pregnancies, the accurate determination of chorionic sex in early pregnancy and selective embryo-reduction surgery to reduce the number of fetuses,can effectively reduce pregnancy complications and improve obstetric outcomes.

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Objective To study the expression and clinical relevance of peripheral blood myeloid-derived suppressor cells(MDSCs) in Acute B lymphoblastic leukemia(B-ALL). Methods Eighty-five patients with confirmed B-ALL and fifteen healthy controls were enrolled. Flow cytometry(FCM) was used to detect the expression of MDSCs in peripheral blood. Results The level of M-MDSCs in the un-remission group was higher than that in the remission group and the healthy control group(P<0.05).The G-MDSCs levels in the un-remission group and the remission group were higher than those in the healthy control group(P<0.05), and the levels of G-MDSCs in the un-remission group were higher than those in the remission group(P<0.05). The expression level of M-MDSCs in high-risk group was higher than that in middle-risk group(P<0.05). B-ALL patients with central invasion had significantly higher G-MDSCs than those without central invasion(P<0.05).Compared with patients with good prognosis, the level of M-MDSCs increased in patients with poor prognosis(P<0.05). In G-MDSCs, poor prognosis and intermediate groups were higher than good prognosis group(P<0.05). Conclusion The level of MDSCs in peripheral blood of patients with B-ALL may be closely related to the occurrence and development of the disease, and it has certain value for judging the prognosis of patients.

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Objective To analyze epidemiology of carbapenem-resistantKlebsiella pnuemoniae(CRKP) among different clones. Methods The phylogenetic relationship was analyzed by multilocus sequence typing(MLST) and the resistant genes were detected by polymerase chain reaction. What′s more, the retrospective analysis was conducted to analyze clinical characteristic of infected patients. Results 13 sequence types(STs) were detected in this study. And ST11 CRKP-infected stains(n=44) were more likely to produceKlebsiella pnuemoniaecarbapenemase(KPC)(P<0.001) compared with non-ST11 ones(n=16), which had higher resistant rate to aminoglycsides(P=0.014). The results of univariate analysis showed that exposure to cephalosporins(P=0.049) and have combined bacteria(P=0.020) prior to infection had close correlations with infection due to ST11 CRKP, but the results of multivariate analysis showed there was no statistical significance. Conclusion Compared with non-ST11-infected strains, ST11 CRKP strains are associated with the production of KPC, but there are no differences in background characteristics and outcomes between groups.

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The subjects were divided into three groups: medical titanium alloy with low elastic modulus, oxidation, medical titanium alloy with low elastic modulus treated by oxidation and coated with tantalum on its surface. The extract was prepared and used to culture mouse fibroblast L929 cells. Flow cytometry method was used to test the cycle distribution and apoptosis of L929 cells and the results were compared by Ti-6 Al-4 V and negative control group 18 ml culture solution containing 10% FBS. After cultured in the extracts for 48 h, the cell proporation of G2 stage in tantalum coatings and Ti-6 Al-4 V group were(14.43±0.18)% and(13.97±1.39)%(P>0.05), the cell apoptosis rate in tantalum coatings and Ti-6 Al-4 V group were(1.39±0.08)% and(1.40±0.05)%(P>0.05). Compared with Ti-6 Al-4 V, medical titanium alloy gradient composites with low elastic modulus had no significant difference in cell cycle and apoptosis of L929 cells, and had good cell compatibility.

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To investigate the safety and effectiveness of laparoscopic surgery for congenital intestinal malrotation and evaluate the value of laparoscopic surgery in the treatment of congenital intestinal malrotation. The clinical data of 183 cases with congenital intestinal malrotation were retrospectively analyzed, including 124 cases of laparoscopic operation and 59 cases of open operation. In patients with intestinal malrotation without midgut volvulus or with midgut volvulus less than 360°, there was no significant difference in operation time, postoperative complication rateand postoperative hospital stay between laparoscopic operation group and open operation group. In patients with intestinal malrotation and midgut volvulus reaching or exceeding 360°, the operation time of laparoscopic surgery group was significantly prolonged, but the postoperative hospital stay was significantly shortened, and the difference was statistically significant. Laparoscopic surgery is safe and effective in the treatment of congenital intestinal malrotation. Although the operation time is prolonged in the group with midgut volvulus reaching or exceeding 360°, postoperative hospital stay is shortened. Laparoscopic surgery has certain advantages and is worth promoting.