〔Abstract〕 Objective To investigate the effects of calcitonin gene-related peptide(CGRP) on osteogenic differentiation and angiogenesis of human periodontal ligament stem cells(hPDLSCs) in vitro. Methods hPDLSCs were isolated and cultured in vitro and identified by flow cytometry. Different concentrations of CGRP were administered,and CCK-8 method was used to detect the effect of CGRP on the proliferation of h PDLSCs. mRNA and protein expression of alkaline phosphatase( ALP),core binding factor 2( RUNX2),osteocalcin( OCN) and vascular endothelial growth factor( VEGF) were detected by RT-PCR and Western blot. ALP staining and alizarin red staining and semi-quantitative analysis were performed on the 7 th and 21 st day of osteogenic induction. Results The cells isolated from human periodontal ligament tissue were identified to be consistent with the characteristics of mesenchymal stem cells. Compared with the 0 mol/L group,CGRP in the 10-8 mol/L group and 10-9 mol/L group promoted the proliferation of h PDLSCs( P<0. 05),and simultaneously increased the expression of ALP,RUNX2,OCN,VEGF gene and protein( P<0. 05). ALP and alizarin red staining and semi-quantitative analysis showed that CGRP in the 10-8 mol/L group and 10-9 mol/L group promoted the expression of ALP and formation of calcium nodules in osteogenic differentiationof h PDLSCs compared with the 0 mol/L group. Conclusion Specific concentrations of CGRP promote osteogenic differentiation and angiogenic ability of h PDLSCs in vitro.