〔Abstract〕 Objective To investigate the correlation between the expression level ofYTHDF1in oral squamous cell carcinoma(OSCC) and clinicopathologic features and its potential prognostic value. Methods The expression ofYTHDF1in 132 OSCC tissues and 66 paracancerous tissues was detected by immunohistochemistry(IHC), and the expression ofYTHDF1protein in OSCC cell lines was detected by Western blot. The correlation betweenYTHDF1and clinicopathological features was analyzed by chi-square test. Kaplan-Meier and Cox factors were used to analyze the factors affecting the survival time of the patients and draw the survival curves of theYTHDF1gene to evaluate its potential clinical significance. Results The expression ofYTHDF1in OSCC tissues was higher than that in paracancerous tissues(P<0.001), and the expression of YTHDF1 protein increased in OSCC cell lines compared with normal oral epithelial keratinocytes(P<0.001). The expression ofYTHDF1was correlated with the TNM stage and T stage of patients with OSCC(P<0.05), and the patients with high expression ofYTHDF1had a shorter survival time compared with those with low expression(P<0.001). Conclusion High expression ofYTHDF1may be associated with poor patient prognosis andYTHDF1may be able to serve as a target for OSCC treatment.