〔Abstract〕 Objective To analyze the characteristics of males with autologous sperm preservation(ASP) in Anhui human sperm bank, and to explore the future direction of ASP in human sperm bank. Methods The basic information of males applied for ASP in Anhui human sperm bank from January 2019 to December 2023 was retrospectively analyzed. Results During this period, there were 424 males applied for ASP. 93.40%(396/424) came from Anhui Province, of which 46.46%(197/424) came from Hefei.The age range of them was 15 to 59 years old. 66.04%(280/424) had a college degree or above. 23.11%(98/424) were employees of public institutions or enterprises. 26.89%(114/424) were unmarried and 89.39%(379/424) were childless. 67.45%(286/424) patients applied for ASP because of assisted reproductive technology treatment. 15.33%(65/424) patients did it due to tumors, among which testicular cancer, lymphoma, leukemia and seminoma were the main reasons.A total of 1 163 semen samples were saved, and 53 males had used their sperm. Conclusion Only a few people applied for ASP, and the characteristics of males with ASP can be used to further strengthen publicity for key groups, especially cancer patients, so as to benefit more people with autologous sperm preservation.