〔Abstract〕 Objective To investigate the expression of Kin of IRRE-like protein 1(KIRREL) in gastric cancer tissues and further explore its effect on angiogenesis. Methods Western blot and IHC were used to detect the expression of KIRREL in gastric cancer tissues and adjacent tissues. Human gastric cancer cell lines SNU-5 and AGS were selected to construct KIRREL-interfering and overexpressing stable cell lines by lentiviral infection of gastric cancer cells. The expression of hypoxia-inducible factor 1α(HIF-1α) and vascular endothelial growth factor(VEGF) in the control, null, interfering and overexpression groups were measured by Western blot, and the effect of KIRREL on the tube-forming ability of human umbilical vein endothelial cells(HUVECs) was observed by angiogenesis assay. Results The expression of KIRREL in gastric cancer tissues was higher than that in adjacent tissues(P<0.05). After silencing KIRREL, the protein expression of HIF-1α and VEGF was significantly reduced, and the tube length and nodes of HUVECs were also significantly reduced; the overexpression of KIRREL was the opposite(P<0.05). Conclusion KIRREL is overexpressed in gastric cancer tissues and may promote angiogenesis in gastric cancer.