〔Abstract〕 Objective To explore the effects of the nerve growth factor neutralizing antibody(anti-NGF) and γ-secretase inhibitor 3,5-difluorophenylacetyl-L-alanyl-S-ph-enylglycine-t-butyl ester(DAPT) on the nuclear expression of p75 neurotrophin receptor(p75NTR) in esophageal cancer Eca109 cells and on cell proliferation and invasion. Methods Immunofluorescence cytochemistry was used to detect the expression changes of p75NTRand Ki67 in Eca109 cells before and after treatment with the anti-NGF and γ-secretase inhibitor DAPT; CCK-8 kit and Transwell cell invasion experiment were used to detect the changes of cell proliferation and invasion ability before and after treatment of Eca109 cells with the anti-NGF and γ-secretase inhibitor DAPT alone or in combination. Results Immunofluorescence cytochemistry showed that after treatment of cells with the anti-NGF and γ-secretase inhibitor DAPT, the number of cells expressing p75NTRin the nucleus decreased, and after the combined treatment of cells with the anti-NGF and γ-secretase inhibitor DAPT, the number of cells expressing p75NTRin the nucleus was the least, and the difference was statistically significant(P<0.05); CCK-8 method and Transwell cell invasion experiment showed that after treatment of cells with the anti-NGF and γ-secretase inhibitor DAPT, cell proliferation and invasion ability were correspondingly weaker than those before treatment, and the difference was statistically significant(P<0.05). Conclusion The anti-NGF and γ-secretase inhibitor DAPT not only inhibit the nuclear transfer of p75NTR, but also reduce the nuclear expression rate of p75NTRand weaken the cell proliferation and invasion ability accordingly.