Found programs:
Authors:Li Chenchen; Xia Rong; Pang Shimeng; Yu Jinlan
Keywords:implant denture;abutment;polydimethylsiloxane;chlorhexidine gluconate;antibacterial properties
DOI:10.19405/j.cnki.issn1000-1492.2022.10.006
〔Abstract〕 Objective To explore the antibacterial coating of polydimethylsiloxane-chlorhexidine gluconate(PDMS-CHXG) constructed on the smooth titanium surface and antibacterial properties forPorphyromonas gingivalisofCHXG solution with different concentrations.Methods The titanium was polished to 7 000 mesh to mirror shape ofabutment, cleaned and dried, then treated with alkalization. The samples were randomly divided into 5 groups:blank control group(C), test groups: grafted CHXG concentration of 0(T0), 0. 4(T1), 0. 8(T2), 1. 6 mg/ml(T3). The surface structural changes were observed by cold field emission scanning electron microscope(CFESEM), and the component elements of coating were analyzed semi-quantitatively. The antibacterial propertiesof the coating were evaluated by antibacterial zone, live/dead bacteria staining and crystal violet experiments.Results A dense film was formed on the surface of titanium under the CFESEM compared with C group. The contentof Cl element increased with the increase of CHXG concentration. There was no inhibition zone around the samplesin C and T0groups, but it was found in T1, T2and T3groups. Live/dead bacteria staining showed no viable bacteria in the T2and T3groups. The results of crystal violet staining showed that T1, T2and T3groups were statistically different from C and T0groups, but the difference between the groups T2and T3was not statistically significant.Conclusion The antibacterial coating of PDMS-CHXG is constructed successfully. The PDMS-CHXG coating dis-plays an exceptional antibacterial property when the concentration of CHXG reaches 0. 8 mg/ml.