〔Abstract〕 Objective To investigate the effects of glycated low density lipoprotein(Gly-LDL) on the growth of human umbilical vein endothelial cells(HUVECs) and the expression of toll like receptor 4(TLR4) and hypoxia inducible factor-1α(HIF-1α), and to explore its possible mechanism. Methods HUVECs were culturedin vitroand divided into control group, positive control group[50 mg/L normal low density lipoprotein(n-LDL)], low concentration, medium concentration and high concentration Gly-LDL(50, 75, 100 mg/L) groups. Respectively, the effects of different concentrations of Gly-LDL on survival rate of HUVECs were detected by CCK-8; The motility of HUVECs under different treatments were detected by wound healing assays; The level of inflammatory cytokine, such as tumor inducing factor-α(TNF-α), interleukin-6(IL-6), intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) were detected by ELISA; The mRNA levels of TLR4, HIF-1α, TNF-α and IL-6 were detected by qRT-PCR; Protein expressions of TLR4, HIF-1α, TNF-α and IL-6 were detected by Western blot; Respectively, si-RNA of TLR4 and HIF-1α was used to intervene the effects of Gly-LDL on HUVECs. The experiment was divided into control group, model group(Gly-LDL 100 mg/L), si-TLR4 group(Gly-LDL 100 mg/L+si-TLR4), TLR4 unloading group(Gly-LDL 100 mg/L+si-NC1), si-HIF-1α group(Gly-LDL 100 mg/L+si-HIF-1α) and HIF-1α unloading group(Gly-LDL 100 mg/L+si-NC2). Protein expressions of TLR4 and HIF-1α were detected by Western blot to verify the interaction between TLR4 and HIF-1α. Results The survival rate and migration rate of HUVECs were inhibited in Gly-LDL(50 mg/L, 75 mg/L, 100 mg/L) group(P<0.01), the inflammatory cytokines, such as TNF-α, IL-6, ICAM-1,VCAM-1 increased by Gly-LDL function on HUVECs(P<0.001), and the mRNA and protein levels of TLR4, HIF-1α, TNF-α and IL-6 increased by Gly-LDL in a dose dependent manner. After TLR4 was knocked out, the proteins expression of TLR4 and HIF-1α were down-regulated compared with model group(P<0.05),but after HIF-1α was knockout, only the protein expression of HIF-1α was down-regulated compared with model group(P<0.01),while the protein expression of TLR4 was up-regulated under the influence of Gly-LDL. Conclusion Gly-LDL may inhibit the proliferation and migration of HUVECs by up-regulating TLR4/HIF-1α inflammatory signaling pathway, and promote the expression of inflammatory cytokines, leading to vascular endothelial injury.