〔Abstract〕 Objective To study the mechanism of di-2-ethylhexyl phthalate(DEHP) in promoting alcoholic fatty liver induced by alcohol combined with high-fat diet. Methods 60 SD male rats were divided into 6 groups randomly, normal group, model group(alcohol), alcohol + DEHP 0.05 mg/kg group, alcohol+DEHP 5 mg/kg group, alcohol+DEHP 500 mg/kg group and DEHP 500 mg/kg control group. The serum and liver tissues of rats were taken, and the levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum were analyzed by biochemistry. The liver injury state of rats was observed by HE slices. The expression levels of P-AMPK and lipin1 protein in liver tissues were detected by Western blot. Results DEHP promoted the accumulation of ALT and AST in serum, aggravated the pathological state of mice induced by alcohol combined with high-fat diet, and at the same time increased lipin-1 protein level and inhibited P-AMPK protein level. Conclusion DEHP may aggravate the pathological process of alcoholic fatty liver rats by mediating lipin-1 and P-AMPK protein levels.