〔Abstract〕 Objective To investigate the relationship between purine metabolism related genesand prognosis of patients with gastric cancer(GC). Methods Ninety-one gastric cancer tissue specimens and 30 paraneoplastic tissue specimens were collected, and the Kyoto Encyclopedia of Genes and Genomes(KEGG) was used to enrich and analyze key genes(KGS) in purine metabolism-related pathways in gastric cancer, and transcriptome sequencing was used to screen differentially expressed genes(DEGs) between gastric cancer tissues and paraneoplastic tissues, and a series of databases were used to overanalyze including Gene Expression Profiling Interactive Analysis(GEPIA) and Kaplan-Meier plotter, to investigate the unique expression and prognostic value of DEGs in gastric cancer patients. Results Eighty-five genes were enriched in the purine metabolic pathway, and differential gene analysis was performed to identify 25 differentially expressed genes, 15 up-regulated genes and 10 down-regulated genes. Five differential genes were further found to be significantly associated with tumor stage in GC. Twenty genes were detected to be closely associated with gastric cancer prognosis. Among them, PDE8 B and PDE9 A were significantly predictive of survival in GC patients with hazard rate(HR) of 1.87 and 1.58. Conclusion Differential genes in the purine metabolic pathway may be potential prognostic markers for gastric cancer, which will provide a new direction for the search of gastric cancer biomarkers.