〔Abstract〕 Objecitve To investigate the preventive effect of ETOH from Asparagus officinalis L. and its different purified componentson carbon tetrachloride(CCl4) induced acute liver injury in mice. Methods Effects of ETOH and its components as petroleum ether extract(PE), ethyl acetate extract(EE), butanol extract(BE) were evaluated by CCl4induced acute liver injury model in mice. Levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bile acid(TBA) and Malondialdehyde(MDA),superoxide dismutase(SOD) in liver tissue as well as the pathology of HE were detected to find out the pharmacodynamic components, with which were further separated and evaluated. Results Levels of serum AST, ALT, TBA, MDA and SOD improved in the group of ETOH and BE(P<0.05), and the effect of BE was better than ETOH. Components of 30% ethanol eluate of butanol extract(BEA), 50% ethanol eluate of butanol extract(BEB), 70% ethanol eluate of butanol extract(BEC) and 90% ethanol eluate of butanol extract(BED) were further purified by BE. Pharmacodynamic evaluation showed that BEA improved the levels of AST, ALT, TBA in serum, and improved MDA and SOD in liver tissue(P<0.05). Content of phenolic acid and flavonoids in BEA was higher compared to that in BE. Conclusion Components named with ETOH, BE and BEA of Oufeng Asparagus officinalis L. can prevent CCl4induced acute liver injury, and the best pharmacodynamic components is BEA, which may be related to the enrichment of flavonoids and phenolic acids.