〔Abstract〕 Objective To prepare nanoparticles containing connective tissue growth factor with poly(L-lactic acid)(PLLA). Methods The connective tissue growth factor-loaded PLLA nanoparticles were prepared by double emulsion method. The characteristics of the nanoparticles were determined, and the morphology of the nanoparticles was observed. Finally, the release behavior of the nanoparticles was examinedin vitro. Results The average diameter of the prepared connective tissue growth factor-loaded PLLA nanoparticles was(376.5±18.2) nm, the incorporation efficiency and drug loading were(68.62±1.34) % and(34.26±0.73) μg/mg, respectively. The nanoparticles showed good spherical morphology without aggregation or adhesion. The release profile consisted of an initial fast release in the first 12 h and a more continuous slow release for 30 d. Conclusion The connective tissue growth factor-loaded PLLA nanoparticles prepared in this study are ideal nanoparticles to be applied on dental implant abutment surface.