〔Abstract〕 Objective To explore a new second-generation mammalian target of rapamycin(mTOR) inhibitor 9-(6-amino-3-pyridyl)-1-[3-(trifluoromethyl) phenyl] benzo[H]-1,6-naphthyridine-2(1 H)-one(Torin2) on the biological behavior of human papillary thyroid carcinoma(PTC) cells, so as to provide a new theoretical basis for clinical targeted treatment of PTC. Methods Human PTC cell line TPC-1 and human normal thyroid cell line Nthy-ori-3 were treated with different concentrations of Torin2. MTT colorimetry was used to detect cell proliferation, cell cycle and apoptosis were analyzed by flow cytometry, and cell migration was analyzed by scratching healing experiment. Results (1) Compared with that before administration, no significant change was observed in the human normal thyroid Nthy-ori-3 cell line;(2) different concentrations Torin2(100, 200, 400 nmol/L)after acting on TPC-1 cells 24, 48 and 72 h, cell proliferation inhibition rate gradually increased, and there was significant difference(P<0.001).And with the extension of action time(24,48,72 h) and the increase of concentration, the inhibition effect on cell proliferation was enhanced;(3)different concentrations Torin2(100, 200, 400 nmol/L)after acting on TPC-1 cells 24 and 48 h, the proportion of G1phase cells gradually increased, while the proportion of S-phase cells gradually decreased, and there was significant difference(P<0.001), indicating significant time and concentration dependence;(4) different concentrations Torin2(100, 200, 400 nmol/L)after acting on TPC-1 cells 24 and 48 h, the apoptosis rate increased significantly and showed a concentration-dependent effect, and there was significant difference(P<0.001);(5) different concentrations Torin2(100, 200, 400 nmol/L)at each concentration could inhibit the migration of TPC-1 cells, and with the increase of the concentration, the inhibition ability was stronger, and there was significant difference(P<0.001). Conclusion Torin2 can significantly inhibit the proliferation and migration of human PTC TPC-1 cells, block cell cycle, induce apoptosis, and enhance its anti-tumor effect with the increase of Torin2 concentration and the prolongation of action time.