〔Abstract〕 Objective To observe cytology differences of liver stem cells(LSCs) in non-inflammatory proliferation environment and inflammatory proliferation environment by establishing non-inflammatory LSCs proliferation model and inflammatory LSCs proliferation model. Methods 2-acetaminofluorenced combined two thirds partial hepatectomy protocol was established as non-inflammatory LSCs proliferation model. 2-acetaminofluorence combined carbon tetrachloride protocol was established as inflammatory LSCs proliferation model. Liver histological changes were observed by hematoxylin and eosin staining. The expressions of EpCAM, CD133 and OV6 of LSCs were detected by immunohistochemistry, and average optical density values were measured by Image-Pro Plus 6.0. The ultrastructure changes of LSCs were observed by transmission electron microscopy. Results Non-inflammatory LSCs proliferation model and inflammatory LSCs proliferation model were established successfully. HE staining:non-inflammatory LSCs proliferation model rats' liver were absent with inflammatory cells infiltration; inflammatory LSCs proliferation model rats' liver were fibrosis formation period with large number of inflammatory cells infiltration. Immunohistochemical: EpCAM-positive, CD133-positive, and OV6-positive LSCs were found both in non-inflammatory and inflammatory LSCs proliferation model, the expression levels of EpCAM, CD133, OV6 of inflammatory LSCs proliferation model were significantly higher than those of non-inflammatory LSCs proliferation model(P<0.05). Transmission electron microscopytype: Ⅰ,Ⅱ, and Ⅲ of LSCs were found both in non-inflammatory and inflammatory LSCs proliferation model, however, there were more heteromorphic LSCs in inflammatory LSCs proliferation model than those in non-inflammatory LSCs proliferation model(P<0.05). Conclusion There are more EpCAM+, CD133+, OV6+LSCs in inflammatory LSCs proliferation model compared with non-inflammatory LSCs proliferation model, and there are LSCs with abnormal subcellular structure in the early stage of hepatitis.